Molecular and Antagonistic Variability of Trichoderma atroviride against Legume Crop Pathogens in Uttar Pradesh, India

Anuradha Singh, Mohammad Shahid, Mukesh Srivastava and S. K. Biswas

  • Page No:  582 - 587
  • Published online: 07 Dec 2013

  • Abstract
  •  samirkrbiswas@rediffmail.com

Antagonistic and molecular variability are reported to exist among eight isolates of Trichoderma atroviride, collected from rhizosphere soil of legumes from different places of Uttar Pradesh, India. Antagonistic variability of isolates of T. atroviride revealed significant suppression in the radial growth of Fusarium oxysporum f.sp. ciceri, F. oxysporum f.sp. lentis and Fusarium udum. The maximum inhibition of 47.91% of mycelial growth of F. udum was recorded in case of TAU8 isolate, which is isolated from soil sample of Azeetmal. TSI3 and TKD3 isolates of Misihinrick and Maitha block of Sitapur and Kanpur dehat, respectively was found (41.66%) least effective against F. udum. Similarly, maximum inhibition of mycelial growth of F. oxysporum f.sp. ciceri was recorded as 55.08% by the TH3 isolate, which was isolated from soil sample of Bilgram block of Hardoi district. The TS5 isolate of Lainbua block of Sitapur was found 39.76% least effective against Fusarium lentis and TH1 showing maximum inhibition of 60.26% myccelial growth against Fusarium lentis. Molecular variability among the isolates showed that total number of amplified bands was found 74 out of which 65 were showing polymorphic and 19 were monomorphic and the size of amplified product varied from 0.1 kb to 0.75 kb.

Keywords :   Variability, mycelial growth, RAPD, Trichoderma atroviride. Fusarium spp.


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