Identification of molecular marker associated with brown plant hopper (Nilaparvata lugens Stal) resistance in rice

R. Selva Pirabu, N. Kumaravadivel, V. Krishnasamy, S. Manonmani, M. Murugan

  • Page No:  334 - 337
  • Published online: 07 Sep 2011

  • Abstract
  •  kumaravadiveln@yahoo.com

In rice, RILs were screened to assess the resistance to brown plant hopper based on the standard seedbox screening test (SSST) in the greenhouse. The parents viz., IR50 and Rathu Heenati had the mean score damage of 5.75 and 3.08, respectively. For the RILs, the leaf damage score ranged from 3.0 to 9.0. As many as 15 SSR primer pairs belonging to chromosome 3 were used to screen the parents for polymorphism. This region was previously reported to be associated with BPH resistance. A total of five polymorphic markers were identified between IR50 and Rathu Heenati. The polymorphic markers were surveyed on 133 RILs to establish their segregation pat­tern. Out of the five marker loci studied, three marker loci viz., RM520, RM3180 and RM6283 showed the expected segregation ratio of 1:1 based on the χ² test at five per cent level. The SSR marker RM2346 was identified to be associated with the BPH resistance based on the damage score from SSST. The SSR marker RM 2346 identified in the present study may be useful in marker-assessed select program after further testing, towards the development of new rice lines with enhanced level of resistance to BPH      

Keywords :  

Rice, Nilaparvata lugens, standard seed box screeing (SSST)


Cite

1.
Pirabu RS, Kumaravadivel N, Krishnasamy V, Manonmani S, Murugan M. Identification of molecular marker associated with brown plant hopper (Nilaparvata lugens Stal) resistance in rice IJBSM [Internet]. 07Sep.2011[cited 8Feb.2022];22011(1):334-337. Available from: http://www.pphouse.org/ijbsm-article-details.php?article=143

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